abstract：:The mechanism of viral RNA replication in mouse hepatitis virus (MHV)-infected cells was studied by oligonucleotide mapping of every mRNA. We discovered that an oligonucleotide, No. 10, was localized at the 5'-end of every mRNA, and was not colinear with the sequences of the virion genomic RNA. This result indicates t...

journal_title：Advances in experimental medicine and biology

authors： Lai MM,Baric RS,Brayton PR,Stohlman SA

更新日期：1984-01-01 00:00:00

abstract：:For several years, the formation and evolution of thrombi in small arteries of rats has been quantitatively studied at the laboratory of PHYSIOLOGY and PHYSIOPATHOLOGY at the V.U.B. Global size parameters can be determined by projecting the image of a small arterial segment onto photosensitive cells. The transmitted l...

journal_title：Advances in experimental medicine and biology

authors： Nyssen M,Blockeel E,Steenhaut O,Bourgain R

更新日期：1984-01-01 00:00:00

abstract：:Single frog skeletal muscle fibres were stretched during fused tetanic contractions. The force increase during stretch exhibited a breakpoint at a mean critical length change of 16.6 nm per half sarcomere that was independent of stretch velocity and sarcomere length. The early decaying extra force after stretch (compo...

journal_title：Advances in experimental medicine and biology

authors： Edman KA,Elzinga G,Noble MI

更新日期：1984-01-01 00:00:00

abstract：:In order to define the ischemic myocardial injury in terms of the possible alteration of cardiac membrane properties, Rb+ uptake and 3H-ouabain binding to cardiac tissue were compared in normal and ischemic rabbit hearts. In isolated Langendorff or working rabbit hearts, a global ischemia was produced by reducing the ...

journal_title：Advances in experimental medicine and biology

authors： Rhee H,Tyler L

更新日期：1984-01-01 00:00:00

abstract：:Laser diffraction and photomicrography have been used to monitor sarcomere length changes in single muscle fibres of the frog, at long sarcomere lengths, during fixed end tetani. In the central 90% of all fibres, changes in sarcomere length were consistently less than 0.25 micron. Sarcomere length showed an initial ra...

journal_title：Advances in experimental medicine and biology

authors： Altringham JD,Pollack GH

更新日期：1984-01-01 00:00:00

abstract：:We measured preretinal oxygen tensions using a microelectrode in dogs made diabetic with alloxan. The intravenous administration of insulin did not affect preretinal oxygen tension over the two hours it could be continuously accurately measured. Furthermore, the oxygen-hemoglobin equilibrium curves measured before and...

journal_title：Advances in experimental medicine and biology

authors： Goldstick TK,Ernest JT,Engerman RL

更新日期：1984-01-01 00:00:00

abstract：:T cell clones are useful models for studying lymphocyte function both at the level of the individual cell and in interacting systems. Murine cytolytic and non- cytolyic T cell clones have been obtained with relative ease, and the particular procedure used to derive and maintain T cell clones may influence profoundly t...

journal_title：Advances in experimental medicine and biology

authors： Fitch FW

更新日期：1984-01-01 00:00:00

abstract：:This retrospective research was conducted in the Department of Obstetrics and Gynaecology of the Dr. Cipto Mangunkusumo Hospital, Jakarta, covering the period between 1977 and 1981. The incidence of hydatidiform mole was 1 in 77 pregnancies. The incidence of malignant trophoblastic disease was 1 in 185 pregnancies. Of...

journal_title：Advances in experimental medicine and biology

authors： Aziz MF,Kampono N,Moegni EM,Sjamsuddin S,Barnas B,Samil RS

更新日期：1984-01-01 00:00:00

abstract：:The effects of adenosine and its derivatives on binding of alpha 2-adrenergic receptor ligands with homogenates of rat vas deferens were examined. Adenosine and 2-chloradenosine increased the amount of [3H]clonidine binding. ATP had a slight effect, but AMP and adenine had none. The effect of adenosine was inhibited b...

journal_title：Advances in experimental medicine and biology

authors： Watanabe Y,Yoshida H

更新日期：1984-01-01 00:00:00

abstract：:Epidemiologic studies have shown that several environmental factors are associated with coronary heart disease (CHD). Most of them are predisposing factors known also as risk factors. Other factors appear to have preventive effects. Blood lipids have been considered the main blood mediator between most of these fac...

journal_title：Advances in experimental medicine and biology

authors： Renaud S

更新日期：1984-01-01 00:00:00

abstract：:We have elaborated the chromosomal editing hypothesis of development. This hypothesis, based on evolutionary arguments, states that the immune system must have evolved from pre-existent cell receptor systems on other tissues. Therefore we feel that what we know about development in the immune system can serve as a pro...

journal_title：Advances in experimental medicine and biology

authors： Dreyer WJ,Roman JM

更新日期：1984-01-01 00:00:00

abstract：:The protective role of alpha 2-macroglobulin, alpha 1-antitrypsin and Aprotinin against trypsin-induced effects on C3 and kininogen was studied in a human in vitro model. When human cationic trypsin was added to human serum or plasma, there was a gradual saturation of alpha 2-macroglobulin and later of alpha 1-antitry...

journal_title：Advances in experimental medicine and biology

authors： Lasson A,Ohlsson K

更新日期：1984-01-01 00:00:00

abstract：:These studies with wild-type and mutant cells defective in IMP dehydrogenase and the previous data with the adenylosuccinate synthetase-deficient cell line suggest that among the clinical population with dominantly inherited hyperuricemia, patients with partial deficiencies in these enzymes exist. It is hoped that the...

journal_title：Advances in experimental medicine and biology

authors： Ullman B

更新日期：1984-01-01 00:00:00

abstract：:Carbon compounds that are needed in small amounts in the diet because they are not made in the body of vertebrates are defined as vitamins. Excluded from this definition are vitamins D, K, and niacin which can be synthesized by the organism or, as in the case of vitamin K, by the host's intestinal bacteria. Lack of su...

journal_title：Advances in experimental medicine and biology

authors： Omaye ST

更新日期：1984-01-01 00:00:00

abstract：:High-field (500 MHz) proton NMR has been used to elucidate the primary and secondary structures of glycosphingolipids (GSLs). Using 2-D J-correlated spectroscopy (2-D SECSY) which establishes scalar couplings of protons, the monosaccharide composition, anomeric configuration and aglycon structures of a GSL can be esta...

journal_title：Advances in experimental medicine and biology

authors： Yu RK,Koerner TA Jr,Demou PC,Scarsdale JN,Prestegard JH

更新日期：1984-01-01 00:00:00

abstract：:Several possibilities are discussed with regard to the biological potency of gangliosides and related compounds as biological response modifier. Evidence is presented that a ganglioside, GQ1b, but not other gangliosides examined exhibits a strong biological activity at a few nanomolar concentration for the promotion o...

journal_title：Advances in experimental medicine and biology

authors： Nagai Y,Tsuji S,Sanai Y

更新日期：1984-01-01 00:00:00

abstract：:The transient length response of the cat papillary muscle in Ba2+-contracture to step tension reduction was found to comprise four different phases. It is tentatively suggested that the transient response is mostly determined by the kinetics of the attachment and detachment of cross-bridges between actin and myosin fi...

journal_title：Advances in experimental medicine and biology

authors： Saeki Y,Shibata T,Sato C,Yanagisawa K

更新日期：1984-01-01 00:00:00

abstract：:By use of an optical system, with which the beam of the first-order diffraction line of He-Ne laser light from a single frog skeletal muscle fiber was split by the wedge-shaped mirror to be focused on two photodiodes ( Haugen & Sten - Knudsen , 1976), small changes in sarcomere length (less than 1 A) could be recorded...

journal_title：Advances in experimental medicine and biology

authors： Sugi H,Kobayashi T

更新日期：1984-01-01 00:00:00

abstract：:The chemical and enzymatic browning reactions of plant polyphenols and their effects on amino acids and proteins are reviewed. A model system of casein and oxidizing caffeic acid has been studied in more detail. The effects of pH, time, caffeic acid level and the presence or not of tyrosinase on the decrease of FDNB-r...

journal_title：Advances in experimental medicine and biology

authors： Hurrell RF,Finot PA

更新日期：1984-01-01 00:00:00

abstract：:The configurations of myosin projections in striated muscles from the marine crab, Portunus trituberculatus were described in the relaxed and rigor states at the full overlap length of the thin and thick filaments. The crystallographic period of the thick filament is 101.5 nm (14.5 nm X 7) and the thick filament has f...

journal_title：Advances in experimental medicine and biology

authors： Wakabayashi K,Namba K,Mitsui T

更新日期：1984-01-01 00:00:00

abstract：:Cinematographic recordings of sarcomere shortening were performed on glycerinated horseshoe crab muscle myofibrils during Ca-activated contraction. When the preparations at slack length (sarcomere length, 7-9 microns) were locally activated with iontophoretically applied Ca ions, the A-band length did not change appre...

journal_title：Advances in experimental medicine and biology

authors： Sugi H,Gomi S

更新日期：1984-01-01 00:00:00

abstract：:In hypoxic rabbits, different levels of Pco2 before and after carotid chemodenervation were applied in order to get information about the acid-base status and the position of the O2-Hb dissociation curve (ODC). A CO2-induced change in pH caused a smaller change in the half-saturation pressure (P50) than was to be expe...

journal_title：Advances in experimental medicine and biology

authors： Kiwull-Schöne H,Gärtner B,Kiwull P

更新日期：1984-01-01 00:00:00

abstract：:Recently, we collected basic morphometric data from normal and hypertrophic rat hearts: median and mean values of the cell diameter and of the intercapillary distance as well as their variabilities. In the present communication we used these data, first for analysis of the effect of the heterogeneity of capillary spac...

journal_title：Advances in experimental medicine and biology

authors： Rakusan K,Hoofd L,Turek Z

更新日期：1984-01-01 00:00:00

abstract：:The myosin-containing A-filaments of vertebrate skeletal muscle contain 294 myosin molecules packed to give overall 3-fold rotational symmetry, as illustrated by the fraying of the filament into 3 sub-filaments ( Maw and Rowe, 1980). Further studies on slightly frayed filaments are consistent with a highly linear arra...

journal_title：Advances in experimental medicine and biology

authors： Rowe AJ,Maw MC

更新日期：1984-01-01 00:00:00

abstract：:The origin of DNA replication of bacteriophage f1 consists of two functional domains: 1) a "core region", about 40 nucleotides long, that is absolutely required for viral (plus) strand replication and contains three distinct but partially overlapping signals, a) the recognition sequence for the viral gene II protein, ...

journal_title：Advances in experimental medicine and biology

authors： Dotto GP,Horiuchi K,Zinder ND

更新日期：1984-01-01 00:00:00

abstract：:We have shown that bovine HMW kininogen remarkably accelerates the activation of Factor XII and prekallikrein in the presence of kaolin, adsorbing on kaolin through the fragment 1.2 region and forming a complex with prekallikrein through the light chain region (Sugo et al., 1980; Ikari et al., 1981). The present study...

journal_title：Advances in experimental medicine and biology

authors： Shimada T,Sugo T,Kato H,Iwanaga S

更新日期：1983-01-01 00:00:00

abstract：:The function and characteristics of mouse folylpolyglutamate synthetase have been examined. Folate polyglutamates were poor substrates for the efflux mechanism for monoglutamates in L1210 mouse leukemia cells, with negligible loss of preformed folate polyglutamates to the medium over four hours. Disruption of folate m...

journal_title：Advances in experimental medicine and biology

authors： Moran RG

更新日期：1983-01-01 00:00:00

abstract：:A collaborative study of the ampouled preparation of porcine pancreatic kininogenase, the Proposed International Reference Preparation (PIRP), was carried out, on behalf of the World Health Organization, by 19 laboratories in seven countries. The PIRP was studied using in vivo bioassays, immunoassays and in vitro enzy...

journal_title：Advances in experimental medicine and biology

authors： Das RE,Hartley RE,Bangham DR

更新日期：1983-01-01 00:00:00

abstract：:The chain length of specific reduced folylpolyglutamates has been estimated by incorporation of the 5,10-methylenetetrahydrofolate form of the cofactor into a stable ternary complex with L. casei thymidylate synthase and tritiated fluorodeoxyuridylate followed by electrophoretic separation based on charge differences ...

journal_title：Advances in experimental medicine and biology

authors： Priest DG,Doig MT

更新日期：1983-01-01 00:00:00

abstract：:Coxsackie B viruses are a common cause of viral myocarditis in humans. A murine model of the human disease has been developed using Coxsackievirus group B, type 3 and inbred Balb/c mice. Infection of T lymphocyte deficient mice does not result in significant myocarditis indicating the importance of T cells in this dis...

journal_title：Advances in experimental medicine and biology

authors： Huber SA,Job LP

更新日期：1983-01-01 00:00:00

abstract：:Kinins and angiotensins affect renal and intestinal function and are rapidly converted and/or inactivated in the renal proximal tubule and intestinal tract by converting enzyme, kininases and angiotensinases of the brush border. Converting enzyme accounts for only 40% of intestinal kininases activity. Thus inhibition ...

journal_title：Advances in experimental medicine and biology

authors： Ward PE,Sheridan MS

更新日期：1983-01-01 00:00:00

abstract：:Changes in tumor tissue oxygenation and acidity were determined using ultramicroelectrodes, and presented in histogram fashion. The effect of Hyperthermia and Hpd photo-therapy were tested. It was found that both modalities affect tumor microcirculation, causing a marked drop in oxygen availability. Tissue pH is decre...

journal_title：Advances in experimental medicine and biology

authors： Bicher HI,Bruley DF

更新日期：1983-01-01 00:00:00

abstract：:It is clear that the combination of 31P NMR studies of muscle and of other organs with biochemical and physiological measurements is an extremely powerful method for studying cellular energetics. We have presented examples of our on-going experiments. Well defined and stable in vitro preparations of mammalian fast-twi...

journal_title：Advances in experimental medicine and biology

authors： Kushmerick MJ,Meyer RA,Brown TR

更新日期：1983-01-01 00:00:00

abstract：:The effect of hypoxia on the in vivo radiation response of tumors is demonstrated both for the measurement of cell survival and growth delay following treatment. Results using these endpoints for the KHT Sarcoma are then presented to demonstrate the effect of various parameters which are expected to control tumor oxyg...

journal_title：Advances in experimental medicine and biology

authors： Hill RP,Pallavicini MG

更新日期：1983-01-01 00:00:00

abstract：:Cultured human fibroblasts accumulated methotrexate polyglutamates to levels far in excess of the dihydrofolate reductase binding capacity. After four days in methotrexate-free medium the intracellular drug level dropped by 70% but nearly 80% of the remaining methotrexate was in the form of polyglutamates. Reduced fol...

journal_title：Advances in experimental medicine and biology

authors： Rosenblatt DS,Whitehead VM

更新日期：1983-01-01 00:00:00

abstract：:Immunization of strain 2 guinea pigs with 10(7) syngeneic Ia+ L2C leukemia cells in adjuvant leads to L2C tumor protection. After subsequent challenges with L2C tumor cells, the sera and spleens of the protected guinea pigs had detectable anti-L2C reactivity. The L2C antibody reactivity was preferentially associated w...

journal_title：Advances in experimental medicine and biology

authors： Ricardo MJ Jr,Grimm DT

更新日期：1983-01-01 00:00:00

abstract：:The glucose metabolism in human polymorphonuclear (PMN) leukocytes was stimulated by latex particles, zymosan particles, gamma-G globulin and formylmet-leu-phe (FMLP) peptide. Maximum stimulation occurred when zymosan particles in amounts above 0.25 mg/ml per 4 X 10(6) leukocytes were used. Latex particles, gamma-G gl...

journal_title：Advances in experimental medicine and biology

authors： Glette J,Bassøe HH

更新日期：1982-01-01 00:00:00

abstract：:The data presented in this paper support a common source for intestinal mast cells in intestinal mucosae. IMC precursors and/or mastocytopoietic factors of as yet unknown origin could be transferred by immune mesenteric lymph node (IMLN) cells of thymus-bearing mice. The differentiated locally or induced mastocytopoie...

journal_title：Advances in experimental medicine and biology

authors： Ruitenberg EJ,Pamentier HK,Elgersma A

更新日期：1982-01-01 00:00:00

abstract：:Lymphocyte development and ontogenetic changes in erythroid cells have been studied in chick-chick yolk sac-embryo chimeras constructed of histoincompatible partners. The results obtained indicate that the early chick yolk sac produces transiently erythroid stem cells whereas definitive erythrocytes are derived from t...

journal_title：Advances in experimental medicine and biology

authors： Toivanen P,Lassila O,Eskola J,Martin C,Dieterlen-Lievre F,Gilmour DG

更新日期：1982-01-01 00:00:00

abstract：:The evidence reviewed here indicates that macrophages, either acting alone or in concert with other cells, influence the proliferation of multiple types of cells. Most of the data indicate that these effects are mediated by soluble macrophage-elaborated products (probably proteins) although the role of direct cell-to-...

journal_title：Advances in experimental medicine and biology

authors： Wharton W,Walker E,Stewart CC

更新日期：1982-01-01 00:00:00